Sunday, February 22, 2009

Memorial Service Program for download

If you missed the service or need another program you can download the Program from his Memorial service. It is a large file. It's a bit different than the one distributed, because there are a few links related to the music performed.

Monday, February 16, 2009

Memorial service slideshow

Here is the slide show of pictures of Fred from the memorial service reception. Thanks to Sally B. Richards for putting this together. The pictures can be browsed and downloaded here. People are encouraged to add comments with descriptions and thoughts on the pictures.

Friday, February 6, 2009

From Melody Lane

This is how I remember Fred – sitting at his desk in KBT, white shirt, sleeves rolled up, ready to work. I was Fred’s secretary from 1971-1981. I was just out of college and he basically trained me to be a scientific secretary. My greatest skill was that I could type fast which was key with Fred as that was the only way to keep up with him. He was in the middle of his chairmanship of the MB&B department. It was then that I learned what a good leader should be. He did this through his charm, wit, cajoling, and even anger. He had no problem yelling at the faculty or staff or administration when he felt it was warranted. He had tremendous energy and enthusiasm for science. During those ten years with him he started the Program-Project Grant with the WERMS groups (Wyckoff, Engelman, Richards, Moore and Steitz) which is still being funded. He started the Research Reports for the department. He was on the Board of the Woods Hole Oceanographic Institute, the Whitney Marine Laboratory, the Advisory Board of Purdue University’s Magnetic Resonance Laboratory, and on the Board of Trustees of Cold Spring Harbor. In 1971 he became a member of the National Academy of Sciences, served on the Council of the International Union of Pure and Applied Biophysics, was President of the American Society of Biochemistry and Molecular Biology, and President of the Biophysical Society, as well as being on the Editorial Boards of the Journal of Molecular Biology and Advances in Protein Chemistry. He carried a full teaching load in an introductory biochemistry course, whose lectures are still taught by other faculty. He carried on a vast correspondence it seemed with everyone in biochemistry, biophysics, the government, and the Hinckley Boatyard. He was on a first-name basis with the University officers. He published extensively and served on many University committees, among which was to chair a committee to establish the Office of Cooperative Research. And he was the Director of the Jane Coffin Childs Fund for Medical Research. And he played hockey at noon! This was all within a ten year span – more than most accomplish in a lifetime. He was incredibly focused and clear in all he did, a very deep thinker. He would dictate correspondence without asking for rough drafts – they were almost always so well thought out that they needed only minor editing; the same for his manuscripts, and grant applications. I found out later how unique that was. His group called him The Chief. I called him Dr. Richards. He had a great team: Johnnie Mouning, Gerry Johnson, Paul Pepin, Art Perlo, all technicians who made the Core work. Fred made it fun to work with him.

Fred had a way of using phrases that I called Fredisms: Get (so-and-so) on the blower for me. Stop grousing and do it. Don’t get into a swivet. I have an appointment with the croaker. He also had a great way of filing which I believe his father taught him. If the pile on your desk gets too big, just take the bottom half and throw it away, because if you haven’t looked for it you don’t need it. Every few months I would hear a big THUMP from his office. It took me a while to catch on to the fact that we were engaged in what I called paper wars. He would spend the morning dictating and then bring out a big stack of papers with the tapes. He’d put them all on my desk with a gleeful smile. The game was on. It was now up to me to get it all done before I left for the day and put it back on HIS desk, so now his desk was cluttered and mine was clean. This is just an example of the challenges he presented in a playful manner. I put a cartoon on his door of a boss speaking to his employee that said “If I wanted it tomorrow, Smedley, I would have given it to you tomorrow.” He loved that.

He was a great communicator. He summed up his packing theory to me by saying, “Put a bunch of stones of different sizes into a bag and leave it – eventually the small stones will go to the bottom and the larger stones will be on top.” Early on we had a brief discussion about who is more creative, an artist or a scientist. His arguments made me aware of just how creative science is and I gained a greater understanding and respect for it. He called it thinking outside the box, which he was known for.

I left the department for about 10 years and came back in 1991, back working for the chairman, a job that Fred had trained me to do years ago. I called him Fred now and he called me Mel and it’s been that way since. Once you work for Fred, you always work for Fred. And it’s been 28 years all told. But we all did it with love and great respect for him. He taught us all so much mostly by example. He even had an uncanny knack for bowing out at the right time – he had no problem handing the controls over to others to let it grow. Even his gruffness was fun because he loved the banter. I think he did it to keep everyone on their toes and keep pushing for excellence. Once he was going on about something and I said he was becoming a curmudgeon – he just smiled broadly, like I finally got it.

Fred was a great leader of the department, even though he had not been chair since 1973. He was always available for advice. You could count on him to really think about an issue and come up with just the right answer. You could trust that he was right. The only thing I can remember that didn’t work for him was his tire jetty project in Little Harbor. I think he would have made it work eventually if not for the neighbors.

One of the last acts as leader was last year when he called a meeting of some of us to put together a scholarship fund for Johnnie Mouning. He was very clear about what he wanted but we were all there as part of his team again to make it all happen. Again, he had an incredible grasp of knowing the right thing to do.

When he had done something he was really proud of, he would call it a thing of beauty and a joy forever. That’s how I remember Fred – a joy forever.

Thursday, February 5, 2009

Obituary Nature Structural & Molecular Biology

Frederic M. Richards, 1925-2009
Wendell A. Lim

Frederic Middlebrook Richards, Sterling Professor Emeritus of Molecular Biophysics and Biochemistry at Yale University, died on January 11, 2009 at the age of 83 (Figure 1). A founding father of the modern field of structural biology and a member of the U.S. National Academy of Sciences, Richards grappled for decades with understanding how proteins fold, and in the process broadly influenced how we determine, analyze and interpret molecular structures today. A master of seeing the forest from the trees, Fred's steady hand helped steer the nascent field forward with logic, common sense, creativity, and a preternatural clarity of vision.

Inspired by a chemist older sister, the young Richards was quickly drawn to the blossoming world of mid-20th century protein chemistry. After obtaining his undergraduate degree in chemistry at MIT in 1948, he entered graduate school in EJ Cohn's Department of Physical Chemistry at Harvard Medical School, where he studied the density and solvent content of protein crystals during his thesis with Barbara Low. In 1954, Richards set off for a short post-doctoral stint with Linderstrom-Lang at the Carlsberg Labs in Denmark, where he was to initiate his classic work on the enzyme ribonuclease.

At that time, bovine pancreatic ribonuclease A (RNase A) was the system of choice for protein chemists, since the Armour Meat Packing company had purified a whopping 1 kg of the protein and offered samples to scientists. At the Carlsberg Labs, Richards experimented with treating RNase A with limiting amounts of the protease subtilisin. He discovered that he could covalently cleave the protein to create a new form, called RNase S, which still retained enzymatic activity. When Richards returned to the U.S., now as a faculty member at Yale, he showed in 1957 that RNaseS could be chromatographically separated into two fragments (referred to as S-protein and S-peptide) each of which lacked any enzymatic activity. But, to the surprise of the protein chemistry community, Richards observed that when he remixed aliquots of the two fragments, complete ribonuclease activity was instantly restored (1, 2). This demonstration that the information encoding the protein's structure and function was robust enough to defy covalent cleavage and physical separation was a landmark finding in the young field of protein folding, and helped set the stage for Anfinsen’s classic experiments demonstrating that RNaseA could spontaneously refold to its native state even after chemical denaturation and scrambling of its disulfide linkages. Richard’s RNaseS work also illustrated the remarkable specificity of protein-protein interactions and how they can regulate protein activity, concepts central to modern cell biology.

Desperately curious to know what these protein structures looked like, Richards played an important role in the beginning days of protein crystallography. In 1967, he and Hal Wykoff solved the structure of RNaseS (3), which was the third protein structure determined (Harker and colleagues also determined the structure of RNase A that year). While on sabbatical in David Phillip's Lab at Oxford, Richards also devised a crucial new method to fit atomic models into electron density maps (4). Prior to this point, Kendrew had used colored clips, placed on a forest of metal rods, to represent electron density. The brass atomic models of the protein were then built within the forest of rods, although this cumbersome setup severely limited the ability to see and to adjust the atomic model. Never one to sit around when presented with an important challenge, Fred set about devising a solution that was typically ingenious in its simplicity. His invention -- the optical comparator (a.k.a. the Richard's Box or "Fred's Folly") -- used a half-silvered mirror placed in the right location relative to a stack of electron density contours and an atomic model (Figure 2). By viewing the device from the proper angle, the user could simultaneously see both the model and the electron density map, and could easily build and adjust the brass atomic pieces so that they fit the density. The Richard's box remained an indispensable tool used by crystallography labs around the world for about 10 years, when it was finally replaced by computer graphics systems. The earliest computer systems were referred to as "electronic Richard's Boxes," and today's programs still use the same basic superposition of electron density maps and atomic models for structure building.

Richards' contributions to protein crystallography also included early experiments that helped the young science weather the skepticism of biochemists who would frequently ask "Why should there be any relation between the structure of a protein in a crystal and in its biologically relevant form in solution?" (5). With Marilyn Doscher and Flo Quiocho, Richards performed decisive experiments demonstrating that enzymes are still active in their crystalline state, thus effectively silencing the nagging doubts and providing support for the crystallographer's leap of faith (6,7). Never tied down to one technique, Fred also made key contributions to the use of NMR and photoaffinity labels to probe molecular structure (8-10). In the later stages of his career, Fred helped shepherd the field as a leader in the movement to require public deposition of published structure coordinates.

Perhaps Richard's longest lasting impact has been on how we think about and interpret structures -- developing the concepts of solvent accessible surface area and internal packing (11). The earliest protein structures yielded complex, disorganized masses that defied the elegant and obvious functional logic of the DNA double helix. Cutting through this complexity, Richards saw the need for simple geometric ways to understand protein structure and function, and in 1971, with BK Lee, developed the concept of solvent accessible surface area -- the surface defined by rolling a sphere the size of solvent over the molecule (Figure 3) (12). This systematic way to determine and quantitate the properties of a protein's "inside" and "outside" as well as the stereochemical properties of the surface that it presents to other potential interaction partners, has played a central role in nearly all studies of protein folding, protein interactions, and the forces that underlie them. Because of the clarity with which molecular surfaces illustrate functional characteristics of a protein, nearly every structural paper today has a molecular surface depiction determined in this basic way.

Richards also quantitatively analyzed the packing within proteins and showed that their interiors were as densely packed as small molecule crystals (11). His thinking on how proteins fold in a manner that solves the puzzle-like problem of close packing their interiors (13) – allowing both maximal occlusion of hydrophobic surface area and optimization of van der Waals interactions -- is central to modern methods in structure prediction and protein design. Current drug design algorithms also trace their origins to ideas of optimizing surface complementarity.

Fred deeply influenced many scientists, beyond his own students and postdocs. At Yale, Fred was the rock upon which the Department of Molecular Biophysics and Biochemisty was built (he was the founding chair in 1967). This department went on to become one of the premier groups in modern structural biology, with seven of the faculty he hired later becoming members of the National Academy of Sciences. Fred, who was often referred to as “the Chief,” led the department with respect and dignity for all. A New England blueblood who could trace his lineage back to the pilgrims, Fred still spoke to everyone with the same square-jawed directness, respect and humor -- whether you were a Nobel laureate, a wide-eyed first year graduate student, or a loyal technician. Despite a sometimes curmudgeonly veneer, it never took long before Fred would break out his famous grin. His renown balanced thinking and sage advice led to his prominent role on diverse scientific advisory committees and as the president of several major scientific societies.

Fred's uncanny ability to home in on the heart of a problem was a key attribute that allowed him to make so many pioneering discoveries and inventions in diverse areas of structural biology. His keen abilities of perception were evident to the generations of Yale students who can recount similar stories: while attending a seminar on a distantly related topic, Fred seemingly dozes off mid-seminar, but awakens as the speaker concludes, only to ask a question that goes straight to the key issue in the field and outlines directions that the field should move in over the next ten years. Because of his clarity of thought, Fred was not often swayed by the distracting forces of dogma and intellectual fashion. Thus, his ideas and opinions always seemed remarkably modern and never outdated.

Perhaps what Fred would have wanted to be remembered for the most was the sheer joy he had for the practice of science. I recall Fred once telling me about how as a young boy, one of his greatest pleasures was convincing his mother to take him to the chemical shops of lower Manhattan, where he would delight in searching for chemicals that might cause pleasing explosions and such. During my postdoc with Fred in the mid 1990's, I witnessed this seventy year-old emeritus professor coming into work each day, giddy with excitement as he sweated at the hood trying to develop a new method to chemically footprint exposed surfaces on proteins (Figure 4) (14). The delight that he took in pursuing his latest creative endeavor was evident. After all of those decades, little had changed -- Fred Richards always knew how to have fun.

Wendell Lim is an HHMI Investigator in the Department of Cellular and Molecular Pharmacology, University of California San Francisco, 600 - 16th Street, San Francisco, CA 94158. email: He was a postdoctoral fellow in Fred Richards' lab from 1992-1996. Much of this material was adapted from an autobiographical review by Richards (4). The author wishes to thank the many admirers of Fred Richards who passed on stories and comments, with special thanks to David Eisenberg (from whom the descriptor "square-jawed directness" is taken), Bob Sauer, Tack Kuntz, Ron Raines, Gerry Olack, Karen Fleming, Melody Lane, and Raghavan Varadarajan. Thanks to Eric Martz for providing pictures of the original Richards optical comparator. References

1. Richards, FM. 1958. On the enzymic activity of subtilisin-modified ribonuclease. Proc. Natl. Acad. Sci USA 44:162-166.

2. Richards, FM , Vithayathil, PJ. 1959. The preparation of subtilisim-modified ribonuclease and the separation of the peptide and protein components. J. Biol. Chem. 234: 1459-1465.

3. Wykoff HW, Hardman KD, Allewell NM, Inagami T, Johnson LN, Richards FM. 1967. the structure of ribonuclease-S at 3.5 Å resolution. J. Biol. Chem. 242:3984-88.

4. Richards FM. 1968. The matching of physical models to three-dimensional electron-density maps: a simple optical device. J. Mol. Biol. 82:1-14.

5. Richards FM. 1997. Whatever happened to the fun? An autobiographical investigation. Annu. Rev. Biophys. Biomol. Struct. 26:1-25.

6. Doscher, MS, Richards FM. 1963. The activity of an enzyme in the crystalline state: Ribonuclease-S. J. Biol. Chem. 238: 2399-406.

7. Quiocho, FA, Richards, FM. 1964. Intermolecular cross-linking of a protein in the crystalline state: carboxypeptidase-A. Proc. Natl. Acad. Sci. USA. 52: 833-839.

8. Lemaster, DM, Richards, FM. 1985. 1H-15N Heteronuclear NMR studies of E. Coli thioredoxin in samples isotopically labeled by residue type. Biochemistry. 24: 7263-7268.

9. Wishart, DS, Sykes BD, Richards, FM. 1991. The relationship between NMR chemical shift and protein structure. J. Mol. Biol. 222: 311-333.

10. Staros, JV, Richards FM. 1974. Photochemical labeling of the surface proteins of human erythocytes. Biochemistry. 13:2720-2726.

11. Richards, FM. 1977 Areas, volume, packing, and protein structure. Ann. Rev. Biophys. Bioeng. 6: 151-176.

12. Lee, B, Richards, FM. 1971. The interpretation of protein structure: estimation of static accessibility. J. Mol. Biol. 55: 379-400.

13. Ponder, JW, Richards, FM. 1987. Tertiary template for proteins - use of packing criteria in the enumeration of allowed sequences for different structural classes. J. Mol. Biol. 193:775-791.

14. Richards, FM, Lamed, R. Wynn, R, Patel, D., Olack, G. 2000. Methylene as a possible universal footprinting reagent that will include hydrophobic surface areas: properties of diazirine as a precursor. Protein Sci. 9:2506-17.

Picture of Fred

Figure 1. Fred Richards with his wife Sally aboard their sailboat Hekla in 1994. A dedicated sailor since childhood, Fred almost always took a month off each summer to captain a major sailing excursion, returning to lab afterwards refreshed and ready to work. His sailing adventures included several transatlantic voyages. He was also an avid ice hockey player (photo by Frank Schley).

Figure 2. The Richards Box: an optical comparator for fitting atomic models to electron density maps. a. The original Richards Box, which was assembled in front of David Philips office at Oxford while Richards was on sabbatical there in 1967-68. The half-silvered mirror for viewing is in the upper left, the atomic model is below, and the electron density map (contours printed on acetates) are in the back (photo from Eric Martz). b. The superimposed image of model and map that the user would see when using the Richards Box (photo from Wendell Lim).

Figure 3. Analyzing protein structures. a. Richards (with BK Lee) developed the concept of solvent accessible surface area. These surfaces were defined by effectively rolling a sphere the size of a water molecule across the protein. This simple geometrical concept allowed a systematic and quantitative analysis of the properties of the "inside" and "outside" of protein structures (image from ref. 11).

b. Richards’ analysis of dense packing within the interior of proteins led to his comparison of protein folding to solving a three-dimensional puzzle (slide from Fred Richards).

Figure 4. Still having fun. a. While in high school at Philips Exeter Academy (1943), Richards and his roommate, John King, attempted to replicate the 1797 Cavendish experiment to measure the earth’s mass and gravitational constant, using a home built apparatus (photo courtesy Don Engelman and David Eisenberg).

b. Richards, still working at the bench in 2004 at the age of 79 (photo by Karen Fleming).